To accomplish this, the serial dilution–agar plate technique is used. Briefly, this method involves serial dilution of a bacterial suspension in sterile water blanks, which serve as a diluent of known volume. Once diluted, the suspensions are placed on suitable nutrient media. The pour-plate technique is the procedure usually employed.
MIC of an antibiotic using broth dilution method is determined by using the following procedure. Preparation of antibiotic stock solution. Preparation of antibiotic dilution range. Preparation of agar dilution plates. Preparation of inoculum. Inoculation. Incubation. Reading and interpreting results.
Jan 17, 2008· Abstract. The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated. MIC values are used to determine susceptibilities of bacteria to drugs.
Abstract. A simplified method has been developed for agar dilution antimicrobial susceptibility testing of anaerobic bacteria, designed to economize on time and money when only a few isolates need to be tested. The procedure is based on the principle of using filter paper disks as carriers of the antibiotic and 35- by 10-mm petri dishes which,.
As an outline to this lecture we are going to talk about susceptibility testing by. dilution methods as part of agar dilution is of course the dilution methods. We are going to go into the particularities and the process and. overview of the process of the testing.
Medium: Plate count agar or Nutrient agar; Procedure for Spread Plate Technique : A: Serial Dilution . Prepare a series of at least 6 test tubes containing 9 ml of sterile distilled water. Using a sterile pipette,add 1ml of sample in the first tube of the set.Label it as 10-1; Mix the contents well by swirling the tube upside down few times.
Agar-dilution susceptibility testing is the reference method for measuring the antimicrobial susceptibilities of strains of N. gonorrhoeae. Resistance to antimicrobial agents is measured as the MIC of the agent that inhibits growth of an isolate.
Agar Dilution Procedure Due to the labor intensive nature of preparation of agar dilution plates, it is suggested that multiple dilutions of the inoculum be tested, especially when time is critical. If the concentration of the organism in the MIC control plate is not 104–105 CFU/ml, the test must be …
The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under deﬁned test conditions, inhibits the visible growth of the bacterium being investigated.
In fact, this method has several advantages compared to the agar diffusion method. The microbroth dilution method is quantitative, allows the use of small quantities of compounds or plant extracts as well as culture media, and is well adapted for drug intended to systemic use (Chung et al., 1995).