– Thin Layer Chromatography (TLC) Guide Overview: Thin Layer Chromatography (TLC) is an extremely useful technique for monitoring reactions. It is also used to determine the proper solvent system for performing separations using column chromatography. TLC uses a stationary phase, usually alumina or silica, that is highly
Experiment 6 . Thin-Layer Chromatography (TLC) OUTCOMES. After completing this experiment, the student should be able to: • explain basic principles of chromatography in general. • describe important aspects of TLC. • identify the composition of an unknown drug mixture by using TLC. DISCUSSION
Chromatography is a separation technique based on difference in polarity of molecules. There are different types of chromatography: paper, thin-layer (TLC), column, high performance liquid (HPLC), and gas chromatography (GC). Chromatography can be used as an analytical technique and/or a preparative technique.
Experiment 6 Fall 2009 2 In column chromatography, the sample is carried down a column of silica or alumina by solvent, and the separate components of the mixture are captured as they elutes from (exit) the column. This can be done by allowing the solvent to flow under the force of gravity, but this is slow.
Start studying CHM 205 | Exp 3a Thin Layer Chromatography. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
Thin Layer Chromatography Procedure. Before starting with the Thin Layer Chromatography Experiment let us understand the different components required to conduct the procedure along with the phases involved. Thin Layer Chromatography Plates – ready-made plates are used which are chemically inert and stable.
In Thin Layer Chromatography ("TLC"), a liquid solution is directly applied to a solid adsorbent. Capillary action draws a developing solvent up the TLC plate. As this solvent passes through the spot, the mixture will be dissolved and will begin to move with the solvent front. However, the adsorbent will also reabsorb part or all of the mixture.
Mar 26, 2014· Thin Layer & Column Chromatography By: Lisa Mickey. Introduction Thin layer chromatography (also known as TLC) is the physical separation of a mixture into its individual components by distributing the components between a stationary phase (the porous TLC plate) and a mobile phase (the solvent that moves through the stationary phase and carries the material that needs …
Colin F. Poole, in The Essence of Chromatography, 2003. 6.1 INTRODUCTION. Thin-layer chromatography (TLC) can trace its origins to the introduction of drop chromatography in the late 1930s [1–4].A microscope slide was covered with a layer of aluminum oxide, on which one drop of extract was spotted, followed by dropwise addition of solvent onto this spot.
This page is an introduction to chromatography using thin layer chromatography as an example. Although if you are a beginner you may be more familiar with paper chromatography, thin layer chromatography is equally easy to describe and more straightforward to explain. Note: I'm taking a …